Home Life Style Kanazawa University research: Understanding a nanomuscle - IHUB Partner Press Releases

Kanazawa University research: Understanding a nanomuscle – IHUB Partner Press Releases

KANAZAWA, Japan, July 14, 2021 /PRNewswire/ — Researchers at Kanazawa University report in Proceedings of the National Academy of Sciences how a protein molecular motor can chop off a piece of cell membrane. The constrict-and-cut mechanism resembles that of a ratchet motor, and is of importance in processes mediating the entry of particles into cells.

Cells in the human body constantly receive substances from the outside via a process called endocytosis. One important endocytosis pathway involves the formation of a protrusion within the cell membrane, pointing to the inside of the cell, triggered when a molecule needing to get in reaches the membrane. The protrusion wraps and closes around the molecule, after which it is cut off, leaving the wrapped molecule (a so-called vesicle) inside the cell. A key role in the cutting-off mechanism is played by dynamin, a protein that can locally ‘constrict’ cell membranes, and chop off bits. The precise constriction mechanism is not completely understood, however. Now, by combining experiments and simulations probing the dynamics of dynamin, Alexander Mikhailov from Kanazawa University and colleagues show that the workings of such a ‘nanomuscle’ resembles that of a ratchet motor.

Dynamin consists of helical filaments, which coil around the neck of a vesicle forming within a membrane. In the presence of GTP (a molecule that can be thought of as a biochemical ‘fuel’, as it provides energy) these filaments start to slide, and in doing so constrict the vesicle neck. In order to gain detailed insights into the constriction mechanism, Mikhailov and colleagues used a technique called single molecule fluorescence resonance energy transfer (smFRET), which enables measuring distances in biomolecules. The obtained data revealed that different conformations of dynamin’s filaments occur, depending on whether or not they are bound to GTP and its products. These various configurations result in the formation of differently oriented molecular ‘bridges’ between neighboring coil turns. Supplying GTP and hydrolyzing it induces power strokes in the cross-bridges and generates a filament torque.

The scientists then designed a computable model of a set of dynamin filaments wrapped around a membrane protrusion, capturing the structural changes and their timescales as observed in the smFRET measurements. The membrane was modelled as a deformable cylindrical tube, and the filaments as strings of beads connected to active cross-bridges powered by GTP. The simulations showed that the combined motion of cross-bridges provides a force large enough to cut a membrane tube.

The work of Mikhalov and colleagues confirms, in a quantitative way, the constriction-by-ratchet model of the dynamin nanomuscle — nature’s strongest torque-generating motor. Quoting the researchers: “Our experimental and computational study provides an example of how collective motor action in megadalton molecular assemblies can be approached and explicitly resolved.”



Dynamin refers to a family of biomolecules. Classical dynamin is a protein playing a key role in endocytosis (the process through which particles are brought into a cell); the molecule is involved in the scission of vesicles formed from a cell membrane. Other molecules from the dynamin family are relevant for various biological processes including the division of organelles and cytokinesis (cell division).

Alexander Mikhailov from Kanazawa University and colleagues have now studied the scission of vesicles by dynamin in detail, and confirmed that when dynamin wraps around a tubular cell membrane fragment, it can constrict it, which results in scission.


Single molecule fluorescence resonance energy transfer (smFRET) is a method used for measuring distances in the nanometer range in single molecules — typically biomolecules.  smFRET is the application of FRET within a single molecule; FRET is the process of energy transfer between two chromophores (molecules that are sensitive to light).  The efficiency of the energy transfer is inversely proportional to the sixth power of the distance between the chromophoric parts, making the FRET technique very sensitive to changes in distance.

Mikhailov and colleagues used smFRET to obtain information on the structure and conformational changes of dynamin, which was an important step in unraveling the constriction mechanism with which dynamin can cut off vesicles from a cell membrane.


Oleg M. Ganichkin, Renee Vancraenenbroeck, Gabriel Rosenblum, Hagen Hofmann, Alexander S. Mikhailov, Oliver Daumke, and Jeffrey K. Noel.  Quantification and demonstration of the collective constriction-by-ratchet mechanism in the dynamin molecular motor, PNAS July 13, 2021 118 (28) e2101144118.

DOI: 10.1073/pnas.2101144118

URL: https://doi.org/10.1073/pnas.2101144118

Image  link


Dynamin as a nanomuscle. The filament coils around a membrane tube and, under GTP hydrolysis, forms active cross-bridges that collectively constrict the membrane.


Hiroe Yoneda
Vice Director of Public Affairs
WPI Nano Life Science Institute (WPI-NanoLSI)
Kanazawa University
Kakuma-machi, Kanazawa 920-1192, Japan
Email: [email protected] 
Tel: +81 (76) 234-4550

About Nano Life Science Institute (WPI-NanoLSI)


Nano Life Science Institute (NanoLSI), Kanazawa University is a research center established in 2017 as part of the World Premier International Research Center Initiative of the Ministry of Education, Culture, Sports, Science and Technology. The objective of this initiative is to form world-tier research centers. NanoLSI combines the foremost knowledge of bio-scanning probe microscopy to establish ‘nano-endoscopic techniques’ to directly image, analyze, and manipulate biomolecules for insights into mechanisms governing life phenomena such as diseases.

About Kanazawa University


As the leading comprehensive university on the Sea of Japan coast, Kanazawa University has contributed greatly to higher education and academic research in Japan since it was founded in 1949. The University has three colleges and 17 schools offering courses in subjects that include medicine, computer engineering, and humanities.

The University is located on the coast of the Sea of Japan in Kanazawa – a city rich in history and culture. The city of Kanazawa has a highly respected intellectual profile since the time of the fiefdom (1598-1867). Kanazawa University is divided into two main campuses: Kakuma and Takaramachi for its approximately 10,200 students including 600 from overseas.

Cision View original content:https://www.prnewswire.com/news-releases/kanazawa-university-research-understanding-a-nanomuscle-301333259.html

SOURCE Kanazawa University

Source link



Please enter your comment!
Please enter your name here

Most Popular

Recent Comments